Redigerer Western blot (avsnitt)

==Uttrykket ''Western blot''==
Uttrykket er dannet i analogi med [[Southern blot]], som er en lignende metode for å detektere DNA. Den metoden ble oppkalt etter sin opphavsmann, den engelske biologen [[Edwin Southern]]. På lignende måte foretar man deteksjon av RNA med en metode som kalles [[Northern blot]].<ref name=Stark1977>{{cite journal |vauthors=Alwine J, Kemp D, Stark G |title=Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes |url=https://archive.org/details/sim_proceedings-of-the-national-academy-of-sciences-usa_1977-12_74_12/page/5350 |journal=Proceedings of the National Academy of Sciences USA |volume=74 |issue=12 |pages=5350–5354 |year=1977 |pmid=414220 |doi=10.1073/pnas.74.12.5350 |pmc=431715|bibcode=1977PNAS...74.5350A }}</ref> Det var W. Neal Burnette som i 1981 begynte å bruke uttrykket «western blot»,<ref name=Burnette1981>{{Cite journal |author=Burnette WN. |title='Western blotting': electrophoretic transfer of proteins from sodium dodecyl sulfate—polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A |journal=Analytical Biochemistry |year=1981 |volume=112 |issue=2 |pages=195–203 |issn=0003-2697 |pmid=6266278 |doi=10.1016/0003-2697(81)90281-5}}</ref> men selve metoden oppstod i 1979 i Harry Towbins laboratorium ved [[Friedrich Miescher Institute for Biomedical Research|Friedrich Miescher Institute]] i [[Basel]] i [[Sveits]].<ref name=Towbin1979>{{cite journal |vauthors=Towbin H, Staehelin T, Gordon J |title=Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications |url=https://archive.org/details/sim_proceedings-of-the-national-academy-of-sciences-usa_1979-09_76_9/page/4350 |journal=Proceedings of the National Academy of Sciences USA |volume=76 |issue=9 |pages=4350–54 |year=1979 |pmid=388439 |doi=10.1073/pnas.76.9.4350 |pmc=411572|bibcode=1979PNAS...76.4350T }}</ref>